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UGT8 Recombinant antibody

UGT8 Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230107A6

Cat No : 82874-1-RR

Print datasheet

Synonyms

Cerebroside synthase, CGT, UDP glycosyltransferase 8, UGT4, UGT8

验证数据展示

  • FC experiment of HeLa using 82874-1-RR

    FC experiment of HeLa using 82874-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human UGT8 (82874-1-RR, Clone:230107A6) and Alexa Fluor™ Plus 647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82874-1-RR targets UGT8 in FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, ELISA
Tested Reactivity Human
Immunogen UGT8 fusion protein Ag13137 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name UDP glycosyltransferase 8
Synonyms Cerebroside synthase, CGT, UDP glycosyltransferase 8, UGT4, UGT8
Calculated Molecular Weight 541 aa, 61 kDa
GenBank Accession NumberBC075069
Gene Symbol UGT8
Gene ID (NCBI) 7368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16880
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells.

实验方案

Product Specific Protocols
FC protocol for UGT8 antibody 82874-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols