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验证数据展示

  • WB analysis of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    WB analysis of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 17982-1-AP (same clone as 17982-1-PBS)

    IHC staining of human brain using 17982-1-AP (same clone as 17982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UGT8 antibody included
  • IHC staining of human brain using 17982-1-AP (same clone as 17982-1-PBS)

    IHC staining of human brain using 17982-1-AP (same clone as 17982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UGT8 antibody included
  • WB analysis of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    WB analysis of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    IP experiment of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    IP result of anti-UGT8 (IP:17982-1-AP, 4ug; Detection:17982-1-AP 1:1000) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UGT8 Polyclonal antibody, PBS Only

UGT8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17982-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

UGT 8, UDP-galactose-ceramide galactosyltransferase, EC:2.4.1.47, CGT, Ceramide UDP-galactosyltransferase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® UGT8 Ready-To-Use IHC Kit. UGT8 primary antibody included.

产品信息

17982-1-PBS targets UGT8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12393

Product name: Recombinant human UGT8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 38-402 aa of BC075069

Sequence: FKTLASALHERGHHTVFLLSEGRDIAPSNHYSLQRYPGIFNSTTSDAFLQSKMRNIFSGRLTAIELFDILDHYTKNCDLMVGNHALIQGLKKEKFDLLLVDPNDMCGFVIAHLLGVKYAVFSTGLWYPAEVGAPAPLAYVPEFNSLLTDRMNLLQRMKNTGVYLISRLGVSFLVLPKYERIMQKYNLLPEKSMYDLVHGSSLWMLCTDVALEFPRPTLPNVVYVGGILTKPASPLPEDLQRWVNGANEHGFVLVSFGAGVKYLSEDIANKLAGALGRLPQKVIWRFSGPKPKNLGNNTKLIEWLPQNDLLGHSKIKAFLSHGGLNSIFETMYHGVPVVGIPLFGDHYDTMTRVQAKGMGILLEWK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP glycosyltransferase 8
别名 UGT 8, UDP-galactose-ceramide galactosyltransferase, EC:2.4.1.47, CGT, Ceramide UDP-galactosyltransferase
计算分子量 541 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC075069
基因名称 UGT8
Gene ID (NCBI) 7368
RRIDAB_2214254
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16880
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells. This antibody specifically recognizes the endogenous UGT8.

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