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UGT3A2 Polyclonal antibody

UGT3A2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21366-1-AP

Print datasheet

Synonyms

UDPGT 3A2, UGT3A2, UNQ842/PRO1780

验证数据展示

  • WB analysis using 21366-1-AP

    WB analysis using 21366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21366-1-AP (UGT3A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 21366-1-AP

    IHC staining of mouse liver using 21366-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21366-1-AP (UGT3A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMOLT-4 cells, human testis tissue, THP-1 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 21366-1-AP is UGT3A2 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Immunogen UGT3A2 fusion protein Ag15835 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP glycosyltransferase 3 family, polypeptide A2
Synonyms UDPGT 3A2, UGT3A2, UNQ842/PRO1780
Calculated Molecular Weight 523 aa, 60 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC103925
Gene Symbol UGT3A2
Gene ID (NCBI) 167127
RRIDAB_10888629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SY77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UGT3A2 antibody 21366-1-APDownload protocol
IHC protocol for UGT3A2 antibody 21366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Front Mol Biosci

Identification of small extracellular vesicle protein biomarkers for pediatric Ewing Sarcoma

Authors - Soumya M Turaga
View Article