验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, mouse kidney tissue, rat colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17268-1-AP targets UGT3A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | UGT3A1 fusion protein Ag11020 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP glycosyltransferase 3 family, polypeptide A1 |
| 别名 | EC:2.4.1.17, UDP-glucuronosyltransferase 3A1, UDPGT 3A1 |
| 计算分子量 | 59 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC035012 |
| 基因名称 | UGT3A1 |
| Gene ID (NCBI) | 133688 |
| RRID | AB_2304309 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6NUS8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UGT3A1 (UDP Glycosyltransferase Family 3 Member A1) belongs to UDP-Glucuronosyltransferases (UGTs) superfamily. UGTs are membrane-bound enzymes localized at the subcellular level in the endoplasmic reticulum (ER) with an active site facing the lumenal side of the ER membrane. The genetic variation in UGT enzymes can modulate its regulation and expression, influencing UGT activity and resulting in pharmacological and physiologic effects. UGT3 family enzymes (UGT3A1 and UGT3A2) are expressed in the thymus, testis, and kidney, with a potential role in the metabolism of ursodeoxycholic acid during the treatment of cholestasis or gallstones.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UGT3A1 antibody 17268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |