验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HEK-293 cells, rat liver tissue |
| Positive IP detected in | rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16661-1-AP targets UGT2B7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag10121 Product name: Recombinant human UGT2B7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-351 aa of BC030974 Sequence: KVLVWAAEYSHWMNIKTILDELIQRGHEVTVLASSASILFDPNNSSALKIEIYPTSLTKTELENFIMQQIKRWSDLPKDTFWLYFSQVQEIMSIFGDITRKFCKDVVSNKKFMKKVQESRFDVIFADAIFPCSELLAELFNIPFVYSLSFSPGYTFEKHSGGFIFPPSYVPVVMSELTDQMTFMERVKNMIYVLYFDFWFEIFDMKKWDQFYSEVLGRPTTLSETMGKADVWLIRNSWNFQFPYPLLPNVDFVGGLHCKPAKPLPKEMEDFVQSSGENGVVVFSLGSMVSNMTEERANVIASALAQIPQKVLWRFDGNKPDTLGLNT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP glucuronosyltransferase 2 family, polypeptide B7 |
| 别名 | 3,4-catechol estrogen-specific UDPGT, EC:2.4.1.17, UDP-glucuronosyltransferase 2B7, UDP-glucuronosyltransferase 2B9, UDPGT 2B7 |
| 计算分子量 | 529 aa, 61 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC030974 |
| 基因名称 | UGT2B7 |
| Gene ID (NCBI) | 7364 |
| RRID | AB_2214249 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P16662 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UDP-glucuronosyltransferase 2B7 (UGT2B7), a member of the UDP-glucuronosyltransferase (UGT) superfamily, mediates phase II biotransformation. During this process, lipophilic substrates undergo glucuronidation conjugation with glucuronic acid, which enhances the aqueous solubility of resultant metabolites and accelerates their elimination via urine or bile (PMID: 10702251; 15231852; 15470161; 15472229). UGT2B7 additionally contributes to the metabolic clearance of mycophenolate, an immunosuppressive pharmaceutical agent (PMID: 15470161).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for UGT2B7 antibody 16661-1-AP | Download protocol |
| IHC protocol for UGT2B7 antibody 16661-1-AP | Download protocol |
| IP protocol for UGT2B7 antibody 16661-1-AP | Download protocol |
| WB protocol for UGT2B7 antibody 16661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Divergent Expression and Metabolic Functions of Human Glucuronosyltransferases through Alternative Splicing. | ||
Br J Pharmacol UDP-Glucuronosyltransferase (UGT)-Mediated Attenuations of Cytochrome P450 3A4 Activity: UGT Isoform-Dependent Mechanism of Suppression. | ||
Sci Rep Inter-isoform Hetero-dimerization of Human UDP-Glucuronosyltransferases (UGTs) 1A1, 1A9, and 2B7 and Impacts on Glucuronidation Activity. | ||
Sci Rep Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues. | ||
Front Pharmacol Use of a Baculovirus-Mammalian Cell Expression-System for Expression of Drug-Metabolizing Enzymes: Optimization of Infection With a Focus on Cytochrome P450 3A4. |
