验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14457-1-AP targets UGT1A10 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | UGT1A10 fusion protein Ag6052 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP glucuronosyltransferase 1 family, polypeptide A10 |
| 别名 | UGT1*10, UDPGT, UDP-glucuronosyltransferase 1-J, UDP-glucuronosyltransferase 1A10, UDP-glucuronosyltransferase 1-10 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC053576 |
| 基因名称 | UGT1A10 |
| Gene ID (NCBI) | 54575 |
| RRID | AB_3669187 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAW8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UGT1A10 belongs to the superfamily of UDP-glucuronosyltransferases (UGTs) catalyzing the glucuronidation reaction, which is important in the detoxification and enhanced elimination of many endogenous and exogenous substrates. Recently, UGT1A10 was the only enzyme in all 19 known human UGTs to catalyze DA glucuronidation. The UGT1As, among three subfamilies (1A, 2A, and 2B) found in human, share a common C-terminal domain of 245 residues. The N-terminal domains that determine their substrate-binding specificity are variable.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UGT1A10 antibody 14457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |