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验证数据展示

  • WB analysis of HepG2 using 86699-1-RR (same clone as 86699-1-PBS)

    WB analysis of HepG2 using 86699-1-RR (same clone as 86699-1-PBS)

    WB result of UGP2 antibody (86699-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UGP2 transfected HepG2 cells. This data was developed using the same antibody clone with 86699-1-PBS in a different storage buffer formulation.

  • WB analysis using 86699-1-RR (same clone as 86699-1-PBS)

    WB analysis using 86699-1-RR (same clone as 86699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86699-1-RR (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86699-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86699-1-RR (same clone as 86699-1-PBS)

    IF Staining of HepG2 using 86699-1-RR (same clone as 86699-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UGP2 antibody (86699-1-RR, Clone: 251615C1 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86699-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86699-1-RR

    Affinity and Kinetic Characterization of 86699-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86699-1-RR against Human UGP2 were performed. The affinity constant is 0.979 nM.

  • Featured Product
  • KD/KO Validated

UGP2 Recombinant monoclonal antibody, PBS Only

UGP2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86699-1-PBS

产品说明书

CloneNo. 251615C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

EC:2.7.7.9, UDPG, UDP-glucose pyrophosphorylase, UDPGP2, UGPase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86699-1-PBS targets UGP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0590

Product name: Recombinant human UGP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-288 aa of BC002954

Sequence: SQDGASQFQEVIRQELELSVKKELEKILTTASSHEFEHTKKDLDGFRKLFHRFLQEKGPSVDWGKIQRPPEDSIQPYEKIKARGLPDNISSVLNKLVVVKLNGGLGTSMGCKGPKSLIGVRNENTFLDLTVQQIEHLNKTYNTDVPLVLMNSFNTDEDTKKILQKYNHCRVKIYTFNQSRYPRINKESLLPVAKDVSYSGENTEAWYPPGHGDIYASFYNSGLLDTFIGEGKEYIFVSNIDNLGATVDLYILNHLMNPPNGKRCEFVMEVTNKTRADVKGGTLTQYE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 UDP-glucose pyrophosphorylase 2
别名 EC:2.7.7.9, UDPG, UDP-glucose pyrophosphorylase, UDPGP2, UGPase
计算分子量 56 kDa
观测分子量 50-56 kDa
GenBank蛋白编号BC002954
基因名称 UGP2
Gene ID (NCBI) 7360
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16851
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379)is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.

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