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UGP2 Monoclonal antibody

UGP2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, Rat, Pig, Rabbit

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G11G6

Cat No : 68381-1-Ig

Print datasheet

Synonyms

pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2

验证数据展示

  • WB analysis using 68381-1-Ig

    WB analysis using 68381-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68381-1-Ig

    WB analysis using 68381-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 68381-1-Ig

    IHC staining of human liver cancer using 68381-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68381-1-Ig (UGP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, pig liver tissue, LNCaP cells, rabbit liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68381-1-Ig targets UGP2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, Rat, Pig, Rabbit samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, Rat, Pig, Rabbit
Immunogen UGP2 fusion protein Ag33160 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name UDP-glucose pyrophosphorylase 2
Synonyms pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC002954
Gene Symbol UGP2
Gene ID (NCBI) 7360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16851
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379) is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.

实验方案

Product Specific Protocols
WB protocol for UGP2 antibody 68381-1-IgDownload protocol
IHC protocol for UGP2 antibody 68381-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols