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验证数据展示

  • WB analysis using 67360-1-Ig (same clone as 67360-1-PBS)

    WB analysis using 67360-1-Ig (same clone as 67360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67360-1-Ig (same clone as 67360-1-PBS)

    IF Staining of HepG2 using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UGDH antibody (67360-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

UGDH Monoclonal antibody, PBS Only

UGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67360-1-PBS

产品说明书

CloneNo. 1E2E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

UDP-GlcDH, UDP-Glc dehydrogenase, UDPGDH, GDH, EC:1.1.1.22

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67360-1-PBS targets UGDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag29747

Product name: Recombinant human UGDH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-301 aa of BC022781

Sequence: MFEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 UDP-glucose dehydrogenase
别名 UDP-GlcDH, UDP-Glc dehydrogenase, UDPGDH, GDH, EC:1.1.1.22
计算分子量 494 aa, 55 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC022781
基因名称 UGDH
Gene ID (NCBI) 7358
RRIDAB_2882612
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO60701
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

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