验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68462-1-Ig targets UGCGL1 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | UGCGL1 fusion protein Ag5940 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | UDP-glucose ceramide glucosyltransferase-like 1 |
| 别名 | FLJ23671, FLJ23796, GT, HUGT1, UGCGL1, UGGT, UGGT1, UGT1, UGTR |
| 计算分子量 | 1531 aa, 175 kDa |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | BC041098 |
| 基因名称 | UGCGL1 |
| Gene ID (NCBI) | 56886 |
| RRID | AB_3670381 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NYU2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UDP-glucose:glycoprotein glucosyltransferase 1 (UGCGL1) is a central quality control gatekeeper in the mammalian endoplasmic reticulum (ER) and serves as a folding sensor in the calnexin/calreticulin glycoprotein quality control cycle (PMID: 28425917). UGCGL1 acts as a checkpoint in the quality control of the MHC class I antigen presentation pathway (PMID: 21383159). Western blot analysis detected UGCGL1at an apparent molecular mass of 170 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UGCGL1 antibody 68462-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |