验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, C6 cells, mouse brain tissue, U-251 cells |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue, mouse brain tissue |
| Positive IF-Fro detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12869-1-AP targets UGCG in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IF-Fro, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3530 Product name: Recombinant human UGCG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-312 aa of BC038711 Sequence: IYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPGYEVAKYDLIWICDSGIRVIPDTLTDMVNQMTEKVGLVHGLPYVADRQGFAATLEQVYFGTSHPRYYISANVTGFKCVTGMSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWRFAMSTQVAMQNSGSYSISQFQSRMIRWTKLRINMLPATIICEPISECFVASLIIGWAAHHVFR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP-glucose ceramide glucosyltransferase |
| 别名 | Glycosylceramide synthase, Glucosylceramide synthase, GLCT-1, GCS, EC:2.4.1.80 |
| 计算分子量 | 394 aa, 45 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC038711 |
| 基因名称 | UGCG |
| Gene ID (NCBI) | 7357 |
| RRID | AB_10915469 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16739 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for UGCG antibody 12869-1-AP | Download protocol |
| IHC protocol for UGCG antibody 12869-1-AP | Download protocol |
| IP protocol for UGCG antibody 12869-1-AP | Download protocol |
| WB protocol for UGCG antibody 12869-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids | ||
Cell Mol Biol Lett UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway | ||
Hum Mol Genet Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase. | ||
iScience Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation. |
