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- KD/KO Validated
UGCG Polyclonal antibody
UGCG Polyclonal Antibody for IF-Fro,IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-Fro,IF-P, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, C6 cells, mouse brain tissue, U-251 cells |
Positive IP detected in | A375 cells |
Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse kidney tissue, mouse brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12869-1-AP targets UGCG in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF-Fro,IF-P, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | UGCG fusion protein Ag3530 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | UDP-glucose ceramide glucosyltransferase |
Synonyms | Ceramide glucosyltransferase, GCS, GLCT 1, Glucosylceramide synthase, UGCG |
Calculated Molecular Weight | 394 aa, 45 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC038711 |
Gene Symbol | UGCG |
Gene ID (NCBI) | 7357 |
RRID | AB_10915469 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16739 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for UGCG antibody 12869-1-AP | Download protocol |
IHC protocol for UGCG antibody 12869-1-AP | Download protocol |
IF protocol for UGCG antibody 12869-1-AP | Download protocol |
IP protocol for UGCG antibody 12869-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids | ||
Hum Mol Genet Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase. | ||
iScience Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation. | ||
Front Pharmacol A Novel Drug Resistance Mechanism: Genetic Loss of Xeroderma Pigmentosum Complementation Group C (XPC) Enhances Glycolysis-Mediated Drug Resistance in DLD-1 Colon Cancer Cells. |