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KD/KO Validated

UFD1L Polyclonal antibody

UFD1L Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF/ICC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat No : 10615-1-AP

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Synonyms

UB fusion protein 1, UFD1, UFD1L

验证数据展示

WB analysis of K-562 using 10615-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 10615-1-AP
IP result of anti-UFD1L (IP:10615-1-AP, 4ug; Detection:10615-1-AP 1:1000) with Raji cells lysate 2000ug.
IF Staining of HeLa using 10615-1-AP
Immunofluorescent analysis of HeLa cells using 10615-1-AP (UFD1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10615-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UFD1L antibody (10615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 10615-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human UFD1L (10615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10615-1-AP

Size

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经过测试的应用

Positive WB detected in	K-562 cells, mouse thymus tissue, Raji cells, HepG2 cells, human testis tissue
Positive IP detected in	Raji cells
Positive IF detected in	HepG2 cells, HeLa cells
Positive FC detected in	HEK-293 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 14 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below

产品信息

10615-1-AP targets UFD1L in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	FC, IF/ICC, IP, WB, ELISA
Cited Applications	WB, IP, IF, IHC
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Immunogen	UFD1L fusion protein Ag0954 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	ubiquitin fusion degradation 1 like (yeast)
Synonyms	UB fusion protein 1, UFD1, UFD1L
Calculated Molecular Weight	35 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC005087
Gene Symbol	UFD1L
Gene ID (NCBI)	7353
RRID	AB_2213944
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92890
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for UFD1L antibody 10615-1-AP	Download protocol
IF protocol for UFD1L antibody 10615-1-AP	Download protocol
IP protocol for UFD1L antibody 10615-1-AP	Download protocol
FC protocol for UFD1L antibody 10615-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IP	Science Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner. Authors - Youngdae Gwon View Article
human	WB	Nature ESCRT-III controls nuclear envelope reformation. Authors - Yolanda Olmos KD Validated View Article
human	WB	Dev Cell The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair. Authors - Samuel S Wallis KD Validated View Article
human	WB	Elife The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP Authors - Maria Körner View Article
mouse	WB	Sci Signal TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing Authors - Liangbin Lin View Article
	WB	Plant J The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development. Authors - Peifeng Yu View Article

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UFD1L Polyclonal antibody

UFD1L Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA

验证数据展示

WB analysis of K-562 using 10615-1-AP

IP experiment of Raji using 10615-1-AP

IF Staining of HeLa using 10615-1-AP

IF Staining of HepG2 using 10615-1-AP

FC experiment of HEK-293 using 10615-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

ESCRT-III controls nuclear envelope reformation.

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP

TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing

The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development.

Flow Cytometry Perm Buffer (10X)