验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
15783-1-AP targets UFC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | UFC1 fusion protein Ag8480 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-fold modifier conjugating enzyme 1 |
| 别名 | HSPC155, UFC1, Ufm1 conjugating enzyme 1 |
| 计算分子量 | 167 aa, 19 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC005187 |
| 基因名称 | UFC1 |
| Gene ID (NCBI) | 51506 |
| RRID | AB_2213938 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3C8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The function of UFC1 (ubiquitin fold modifier conjugating enzyme 1) has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein. Catalog#15783-1-AP specially recognises the 21 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UFC1 antibody 15783-1-AP | Download protocol |
| IHC protocol for UFC1 antibody 15783-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
Mol Cell UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity
| ||
Cancer Lett CDK5RAP3 is a novel super-enhancer-driven gene activated by master TFs and regulates ER-Phagy in neuroblastoma | ||
BMC Cardiovasc Disord Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice |
