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验证数据展示

  • WB analysis using RMX00036

    WB analysis using RMX00036

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00036 (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using RMX00036

    IF Staining of mouse brain using RMX00036

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UCP1 antibody (RMX00036) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brown adipose using RMX00036

    IP experiment of mouse brown adipose using RMX00036

    IP result of anti-UCP1 (IP:RMX00036, 4ug; Detection:RMX00036 1:10000) with mouse brown adipose tissue lysate 1280 ug.

  • IHC staining of mouse brown adipose using RMX00036

    IHC staining of mouse brown adipose using RMX00036

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using RMX00036 (UCP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brown adipose using RMX00036

    IHC staining of mouse brown adipose using RMX00036

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using RMX00036 (UCP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Multi-rAb® UCP1 Multi-Recombinant antibody

UCP1 Multi-Recombinant Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. RMX00036

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IP, ELISA

别名

SLC25A7, Thermogenin, UCP, UCP 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brown adipose tissue
Positive IP detected inmouse brown adipose tissue
Positive IHC detected inmouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00036 targets UCP1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

Mixture

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 uncoupling protein 1 (mitochondrial, proton carrier)
别名 SLC25A7, Thermogenin, UCP, UCP 1
计算分子量 307 aa, 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC098168
基因名称 UCP1
Gene ID (NCBI) 7350
RRIDAB_3750090
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP25874
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

实验方案

Product Specific Protocols
IF protocol for UCP1 antibody RMX00036Download protocol
IHC protocol for UCP1 antibody RMX00036Download protocol
IP protocol for UCP1 antibody RMX00036Download protocol
WB protocol for UCP1 antibody RMX00036Download protocol
Standard Protocols
Click here to view our Standard Protocols
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