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验证数据展示

  • WB analysis using 84380-1-RR (same clone as 84380-1-PBS)

    WB analysis using 84380-1-RR (same clone as 84380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

    IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

    IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

Urocortin Recombinant monoclonal antibody, PBS Only

Urocortin Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84380-1-PBS

产品说明书

CloneNo. 241183B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

UCN, UROC, UI, 241183B12

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84380-1-PBS targets Urocortin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 urocortin
别名 UCN, UROC, UI, 241183B12
计算分子量 13 kDa
观测分子量22 kDa
GenBank蛋白编号NM_003353
基因名称 UCN
Gene ID (NCBI) 7349
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP55089
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).

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