验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, HEK-293 cells, HeLa cells, human kidney tissue, human testis tissue |
Positive IP detected in | mouse kidney tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
19731-1-AP targets Urocortin in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | urocortin |
别名 | UCN, UROC, UI |
计算分子量 | 13 kDa |
观测分子量 | 22 kDa |
GenBank蛋白编号 | NM_003353 |
基因名称 | UCN |
Gene ID (NCBI) | 7349 |
RRID | AB_10640909 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P55089 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).
实验方案
Product Specific Protocols | |
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WB protocol for Urocortin antibody 19731-1-AP | Download protocol |
IP protocol for Urocortin antibody 19731-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |