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UCKL1 Polyclonal antibody

UCKL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17005-1-AP

Print datasheet

Synonyms

F538, UCK1 LIKE, UCK1L, UCKL1, Uridine cytidine kinase like 1, URKL1

验证数据展示

  • WB analysis of K-562 using 17005-1-AP

    WB analysis of K-562 using 17005-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 17005-1-AP

    IHC staining of mouse kidney using 17005-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 17005-1-AP

    IHC staining of mouse kidney using 17005-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, Raji cells, rat kidney tissue, U-937 cells
Positive IHC detected inmouse kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17005-1-AP targets UCKL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen UCKL1 fusion protein Ag10701 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uridine-cytidine kinase 1-like 1
Synonyms F538, UCK1 LIKE, UCK1L, UCKL1, Uridine cytidine kinase like 1, URKL1
Calculated Molecular Weight 419 aa, 46 kDa
Observed Molecular Weight 61 kDa, 46 kDa
GenBank Accession NumberBC033078
Gene Symbol UCKL1
Gene ID (NCBI) 54963
RRIDAB_2210783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWZ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UCKL1 antibody 17005-1-APDownload protocol
IHC protocol for UCKL1 antibody 17005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

EBioMedicine

Non-canonical role of UCKL1 on ferroptosis defence in colorectal cancer

Authors - Weili Wu
  • KD Validated
View Article