验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, RT-4 cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32722-1-AP targets UBXN7 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38516 Product name: Recombinant human UBXN7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-200 aa of NM_015562.1 Sequence: LDGGGIAEEPSTSSASVSTVRPHTEEEVRAPIPQKQEILVEPEPLFGAPKRRRPARSIFDGFRDFQTETIRQEQELRNGGAIDKKLTTLADLFRPPIDLMHKGSFETAKECGQMQNKWLMINIQNVQDFACQCLNRDVWSNEAVKNIIREH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UBX domain protein 7 |
| 别名 | KIAA0794, UBX domain-containing protein 7, UBXD7 |
| 计算分子量 | 55kDa，489aa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | NM_015562.1 |
| 基因名称 | UBXN7 |
| Gene ID (NCBI) | 26043 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O94888 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBXN7(UBX domain protein 7) is a protein with ubiquitin-binding activity and ubiquitin-proteasome-binding activity. It contains four different domains, namely, the N-terminal UBA domain, the UAS domain, the Ubiquitin Interaction motif (UIM) and the C-terminal UBX domain. The level of UBXN7 will affect the metabolic state of cells. High levels of UBXN7 and HIF-1α accumulation support glycolysis, while inactivation of UBXN7 and activation of NRF2 increase oxidative phosphorylation (PMID: 33444648).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBXN7 antibody 32722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
