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验证数据展示

  • WB analysis using 25141-1-AP (same clone as 25141-1-PBS)

    WB analysis using 25141-1-AP (same clone as 25141-1-PBS)

    Neuro-2a cells lysates were subjected to SDS PAGE followed by western blot with 25141-1-AP (UBXN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 25141-1-AP (same clone as 25141-1-PBS)

    IHC staining of human ovary cancer using 25141-1-AP (same clone as 25141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25141-1-AP (UBXN2B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 25141-1-AP (same clone as 25141-1-PBS)

    IF Staining of Neuro-2a using 25141-1-AP (same clone as 25141-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UBXN2B antibody (25141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25141-1-PBS in a different storage buffer formulation.

UBXN2B Polyclonal antibody, PBS Only

UBXN2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25141-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25141-1-PBS targets UBXN2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18259

Product name: Recombinant human UBXN2B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-170 aa of BC113645

Sequence: MAEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHEYSGLNIVRPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQDVQILLKLWSNGFSLDDGELRPYNEPTNAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UBX domain protein 2B
别名 UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37
计算分子量 331 aa, 37 kDa
观测分子量37 kDa
GenBank蛋白编号BC113645
基因名称 UBXN2B
Gene ID (NCBI) 137886
RRIDAB_3085772
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14CS0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBXN2B, also called p37, is a cofactor of the p97 AAA ATPase. UBXN2B regulates spindle orientation during mitosis by limiting the levels of cortical NuMA (PMID: 29222185).

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