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- KD/KO Validated
UBXN1 Polyclonal antibody
UBXN1 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, WB,ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human liver tissue, mouse cerebellum tissue, human brain tissue, human heart tissue, HepG2 cells, Ramos cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:30000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16135-1-AP targets UBXN1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, WB,ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Immunogen | UBXN1 fusion protein Ag9146 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | UBX domain protein 1 |
| Synonyms | 2B28, LOC51035, SAKS1, SAPK substrate protein 1, UBA/UBX 33.3 kDa protein, UBX domain protein 1, UBXD10, UBXN1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC000902 |
| Gene Symbol | UBXN1 |
| Gene ID (NCBI) | 51035 |
| RRID | AB_2210480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04323 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBXN1 antibody 16135-1-AP | Download protocol |
| IHC protocol for UBXN1 antibody 16135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins. | ||
Cancer Lett The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells. | ||
Front Immunol PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma. | ||
Mol Cell Biol The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.
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