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验证数据展示

  • WB analysis of human liver using 13172-1-AP

    WB analysis of human liver using 13172-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13172-1-AP (UBTD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13172-1-AP

    IHC staining of human breast cancer using 13172-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13172-1-AP (UBTD2 antibody) at dilution of 1:100 (under 10x lens).

UBTD2 Polyclonal antibody

UBTD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13172-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DCUBP, DC-UbP, ubiquitin domain containing 2, Ubiquitin like protein SB72, UBTD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13172-1-AP targets UBTD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原

CatNo: Ag3827

Product name: Recombinant human UBTD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC019910

Sequence: DSSGSLNENSEGTGVALGRNQPLKKEKPKWKSDYPMTDGQLRSKRDEFWDTAPAFEGRKEIWDALKAAAHAFESNDHELAQAIIDGANITLPHGALTECYDELGNRYQLPVYCLAPPINMIEEKSDIETLDIPEPPPNSGYECQLRLRLSTGKDLKLVVRSTDTVFHMKRRLHAAEGVEPGSQRWFFSGRPLTDKMKFEELKIPKDYVVQVIVSQPVQNPTPVEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin domain containing 2
别名 DCUBP, DC-UbP, ubiquitin domain containing 2, Ubiquitin like protein SB72, UBTD2
计算分子量 234 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC019910
基因名称 UBTD2
Gene ID (NCBI) 92181
RRIDAB_2210317
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WUN7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dendritic cell-derived ubiquitin (Ub)-like protein (DC-UbP), also named as Ub domain-containing protein 2 (UBTD2), is a potential Ub shuttle protein comprised of a Ub-like (UbL) domain and a Ub-binding domain (UBD), but its biological function remains largely unknown.PMID: 25207809

实验方案

Product Specific Protocols
WB protocol for UBTD2 antibody 13172-1-APDownload protocol
IHC protocol for UBTD2 antibody 13172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Ann N Y Acad Sci

miR-141-3p affects β-catenin signaling and apoptosis by targeting Ubtd2 in rats with anorectal malformations

Authors - Chen Yi Wang
View Article