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UBR1 Polyclonal antibody

UBR1 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 26069-1-AP

Print datasheet

Synonyms

JBS, N recognin 1, UBR1

验证数据展示

  • IP experiment of mouse skeletal muscle using 26069-1-AP

    IP experiment of mouse skeletal muscle using 26069-1-AP

    IP result of anti-UBR1 (IP:26069-1-AP, 4ug; Detection:26069-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

  • IHC staining of human pancreas using 26069-1-AP

    IHC staining of human pancreas using 26069-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26069-1-AP (UBR1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 26069-1-AP

    IHC staining of human pancreas using 26069-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26069-1-AP (UBR1 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26069-1-AP targets UBR1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen UBR1 fusion protein Ag22076 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin protein ligase E3 component n-recognin 1
Synonyms JBS, N recognin 1, UBR1
Calculated Molecular Weight 1749 aa, 200 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC113505
Gene Symbol UBR1
Gene ID (NCBI) 197131
RRIDAB_2880360
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWV7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for UBR1 antibody 26069-1-APDownload protocol
IP protocol for UBR1 antibody 26069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB,IP

J Cachexia Sarcopenia Muscle

Identification of Ubr1 as an amino acid sensor of steatosis in liver and muscle

Authors - Wanni Zhao
View Article
humanWB

Diabetes

N-end Rule-Mediated Proteasomal Degradation of ATGL Promotes Lipid Storage

Authors - Jiesi Xu
  • KD Validated
View Article