验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
16400-1-AP targets UBQLNL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | UBQLNL fusion protein Ag9496 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquilin-like |
| 别名 | Ubiquilin-like protein, Ubiquilin like protein, ubiquilin like |
| 计算分子量 | 475 aa, 53 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC012183 |
| 基因名称 | UBQLNL |
| Gene ID (NCBI) | 143630 |
| RRID | AB_2878253 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IYU4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBQLNL also named as Ubiquilin like protein, encodes a 475 amino acid protein. The specific function of it is still non-known. UBQLNL may have an association with type 2 diabetes, but the mechanism is supposed be studied in the further research. The subcellular localization of UBQLNL is nucleus. The expression of it is detected in some tissues, such as brain ,testis, heart and liver.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBQLNL antibody 16400-1-AP | Download protocol |
| IHC protocol for UBQLNL antibody 16400-1-AP | Download protocol |
| IF protocol for UBQLNL antibody 16400-1-AP | Download protocol |
| IP protocol for UBQLNL antibody 16400-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
