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验证数据展示

  • WB analysis of mouse testis using 23372-1-AP

    WB analysis of mouse testis using 23372-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23372-1-AP

    IHC staining of human testis using 23372-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50.

  • IHC staining of human testis using 23372-1-AP

    IHC staining of human testis using 23372-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50.

  • IF Staining of HepG2 using 23372-1-AP

    IF Staining of HepG2 using 23372-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN3 antibody (23372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

UBQLN3 Polyclonal antibody

UBQLN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23372-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

UBQLN 3, Ubiquilin-3, ubiquilin3, ubiquilin 3, TUP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, HepG2 cells, human testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23372-1-AP targets UBQLN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19982

Product name: Recombinant human Ubiquilin 3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 306-620 aa of BC036743

Sequence: WTSTHGGSGSRQGRQDGDQDAPDIRNRFPNFLGIIRLYDYLQQLHENPQSLGTYLQGTASALSQSQEPPPSVNRVPPSSPSSQEPGSGQPLPEESVAIKGRSSCPAFLRYPTENSTGQGGDQDGAGKSSTGHSTNLPDLVSGLGDSANRVPFAPLSFSPTAAIPGIPEPPWLPSPAYPRSLRPDGMNPAPQLQDEIQPQLPLLMHLQAAMANPRALQALRQIEQGLQVLATEAPRLLLWFMPCLAGTGSVAGGIESREDPLMSEDPLPNPPPEVFPALDSAELGFLSPPFLHMLQDLVSTNPQQLQPEAHFQVQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquilin 3
别名 UBQLN 3, Ubiquilin-3, ubiquilin3, ubiquilin 3, TUP1
计算分子量 655 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC036743
基因名称 UBQLN3
Gene ID (NCBI) 50613
RRIDAB_2879268
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H347
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBQLN3(Ubiquilin-3) may interact with the testis-specific cyclin A1 and regulate cell cycle progression during spermatogenesis, as does frog Xdrp1, with which UBQLN3 shares sequence similarity(PMID:10831842).UBQLN3 expression is not observed in mouse eye, thyroid, pancreas,smooth muscle, ovary, submaxillary gland, uterus, and epididymis by northern blot(PMID:10831842 ).

实验方案

Product Specific Protocols
IF protocol for UBQLN3 antibody 23372-1-APDownload protocol
IHC protocol for UBQLN3 antibody 23372-1-APDownload protocol
WB protocol for UBQLN3 antibody 23372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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