验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, rat brain tissue, HeLa cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23449-1-AP targets Ubiquilin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, ELISA |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag20149 Product name: Recombinant human Ubiquilin 2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-624 aa of BC069237 Sequence: MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquilin 2 |
| 别名 | UBQLN2, ALS15, Chap1, Dsk2, hPLIC 2 |
| 计算分子量 | 624 aa, 66 kDa |
| 观测分子量 | 66-70 kDa |
| GenBank蛋白编号 | BC069237 |
| 基因名称 | UBQLN2 |
| Gene ID (NCBI) | 29978 |
| RRID | AB_2879282 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHD9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquilin-2 (UBQLN2) is a multifunctional, ubiquitin-binding shuttle protein that plays a central role in maintaining cellular protein homeostasis. It is a member of the ubiquilin family of proteins found in all eukaryotes, which function to regulate the degradation of ubiquitinated proteins. Of the five different UBQLN genes in humans, UBQLN2 is the most highly expressed in the nervous system and muscle tissue and has been linked to multiple neurodegenerative diseases. (PMID: 40663766, PMID: 40663766)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Ubiquilin 2 antibody 23449-1-AP | Download protocol |
| IHC protocol for Ubiquilin 2 antibody 23449-1-AP | Download protocol |
| WB protocol for Ubiquilin 2 antibody 23449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Tamoxifen improves muscle structure and function of Bin1- and Dnm2-related centronuclear myopathies | ||
Hum Mol Genet Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration. | ||
J Neurochem Pathogenic mutation of UBQLN2 impairs its interaction with UBXD8 and disrupts endoplasmic reticulum-associated protein degradation. | ||
F1000Res The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
| ||
bioRxiv Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4 |
