验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HUVEC cells, mouse testis tissue, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14253-1-AP targets UBL4A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag5526 Product name: Recombinant human UBL4A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-157 aa of BC043346 Sequence: MQLTVKALQGRECSLQVPEDELVSTLKQLVSEKLNVPVRQQRLLFKGKALADGKRLSDYSIGPNSKLNLVVKPLEKVLLEEGEAQRLADSPPPQVWQLISKVLARHFSAADASRVLEQLQRDYERSLSRLTLDDIERLASRFLHPEVTETMEKGFSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-like 4A |
| 别名 | Ubiquitin-like protein 4A, ubiquitin like 4A, GDX, G6PD, DXS254E |
| 计算分子量 | 18 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC043346 |
| 基因名称 | UBL4A |
| Gene ID (NCBI) | 8266 |
| RRID | AB_2288205 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11441 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for UBL4A antibody 14253-1-AP | Download protocol |
| IHC protocol for UBL4A antibody 14253-1-AP | Download protocol |
| IP protocol for UBL4A antibody 14253-1-AP | Download protocol |
| WB protocol for UBL4A antibody 14253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer | ||
J Immunol UBL4A Augments Innate Immunity by Promoting the K63-Linked Ubiquitination of TRAF6.
| ||
J Nutr Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs. | ||
J Inherit Metab Dis Mutations in GET4 disrupt the transmembrane domain recognition complex pathway. | ||
Pediatr Res Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets |
