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- KD/KO Validated
UBL4A Polyclonal antibody
UBL4A Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HUVEC cells, mouse testis tissue, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14253-1-AP targets UBL4A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Immunogen | UBL4A fusion protein Ag5526 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin-like 4A |
| Synonyms | DX254E, DXS254E, G6PD, GDX, ubiquitin like 4A, Ubiquitin like protein 4A, Ubiquitin like protein GDX, UBL4, UBL4A |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC043346 |
| Gene Symbol | UBL4A |
| Gene ID (NCBI) | 8266 |
| RRID | AB_2288205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11441 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBL4A antibody 14253-1-AP | Download protocol |
| IHC protocol for UBL4A antibody 14253-1-AP | Download protocol |
| IF protocol for UBL4A antibody 14253-1-AP | Download protocol |
| IP protocol for UBL4A antibody 14253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunol UBL4A Augments Innate Immunity by Promoting the K63-Linked Ubiquitination of TRAF6.
| ||
J Nutr Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs. | ||
J Inherit Metab Dis Mutations in GET4 disrupt the transmembrane domain recognition complex pathway. | ||
Pediatr Res Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets | ||
J Exp Clin Cancer Res UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1.
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