验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, human brain tissue, U-87 MG cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
14100-1-AP targets UBL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag5233 Product name: Recombinant human UBL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-117 aa of BC059385 Sequence: MSSNVPADMINLRLILVSGKTKEFLFSPNDSASDIAKHVYDNWPMDWEEEQVSSPNILRLIYQGRFLHGNVTLGALKLPFGKTTVMHLVARETLPEPNSQGQRNREKTGESNCCVIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-like 3 |
| 别名 | HCG 1, HsMUB, MUB, PNSC1, Protein HCG 1, ubiquitin like 3, Ubiquitin like protein 3, UBL3 |
| 计算分子量 | 117 aa, 13 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC059385 |
| 基因名称 | UBL3 |
| Gene ID (NCBI) | 5412 |
| RRID | AB_2212060 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95164 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin-like 3 (UBL3)/membrane-anchored Ub-fold protein (MUB) acts as a post-translational modification (PTM) factor to regulate efficient protein sorting to sEVs (small extracellular vesicles). (PMID: 31363817, PMID: 30258067).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBL3 antibody 14100-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biol Open Intracellular dynamics of ubiquitin-like 3 visualized using an inducible fluorescent timer expression system | ||
Mol Brain Comprehensive identification of ubiquitin-like 3 (UBL3)-interacting proteins in the mouse brain |
