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Species Reactivity
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验证数据展示

  • WB analysis using 32161-1-AP

    WB analysis using 32161-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32161-1-AP (UBFD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

UBFD1 Polyclonal antibody

UBFD1 Polyclonal Antibody for WB, ELISA
Cat No. 32161-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Ubiquitin-binding protein homolog, Ubiquitin Family Domain Containing 1 , Ubiquitin domain-containing protein UBFD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32161-1-AP targets UBFD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 UBFD1 fusion protein Ag36361 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin family domain containing 1
别名 Ubiquitin-binding protein homolog, Ubiquitin Family Domain Containing 1 , Ubiquitin domain-containing protein UBFD1
计算分子量309aa, 33 kDa
观测分子量40 kDa
GenBank蛋白编号NM_019116
基因名称 UBFD1
Gene ID (NCBI) 56061
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO14562
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBFD1 (Ubiquitin Family Domain Containing 1) is a polyubiquitin-binding protein containing ubiquitin-like structural domains that are widely involved in protein degradation and cell signaling. This protein shows aberrant expression in a variety of cancers, especially in estrogen receptor (ER)-positive breast cancer, where high expression of UBFD1 is closely associated with poor prognosis of patients, and is considered as a potential prognostic biomarker and therapeutic target. In addition, UBFD1 was also found to be associated with MYC-mediated tumor aggressiveness in T-cell acute lymphoblastic leukemia (T-ALL), which functionally involves the endoplasmic reticulum-associated degradation (ERAD) pathway. UBFD1 is also expressed in inner ear hair cells and ear epithelial cells, suggesting that it may play a role in hearing-related diseases.

实验方案

Product Specific Protocols
WB protocol for UBFD1 antibody 32161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols