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UBF1 Polyclonal antibody

UBF1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20660-1-AP

Print datasheet

Synonyms

Autoantigen NOR 90, NOR 90, UBF, UBF 1, UBF1, UBTF, Upstream binding factor 1

验证数据展示

  • WB analysis of HT-1080 using 20660-1-AP

    WB analysis of HT-1080 using 20660-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20660-1-AP

    IF Staining of HepG2 using 20660-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 20660-1-AP (UBF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHT-1080 cells, HepG2 cells, MCF-7 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20660-1-AP targets UBF1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name upstream binding transcription factor, RNA polymerase I
Synonyms Autoantigen NOR 90, NOR 90, UBF, UBF 1, UBF1, UBTF, Upstream binding factor 1
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberNM_014233
Gene Symbol UBTF
Gene ID (NCBI) 7343
RRIDAB_2878718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17480
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)

实验方案

Product Specific Protocols
WB protocol for UBF1 antibody 20660-1-APDownload protocol
IF protocol for UBF1 antibody 20660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols