验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, HepG2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20660-1-AP targets UBF1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | upstream binding transcription factor, RNA polymerase I |
| 别名 | UBTF, UBF-1, UBF 1, UBF, NOR 90 |
| 计算分子量 | 89 kDa |
| 观测分子量 | 85-90 kDa |
| GenBank蛋白编号 | NM_014233 |
| 基因名称 | UBF1 |
| Gene ID (NCBI) | 7343 |
| RRID | AB_2878718 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17480 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBF1 antibody 20660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
