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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 85332-2-RR (same clone as 85332-2-PBS)

    WB analysis using 85332-2-RR (same clone as 85332-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85332-2-RR (UBE4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85332-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85332-2-RR (same clone as 85332-2-PBS)

    IF Staining of HeLa using 85332-2-RR (same clone as 85332-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE4B antibody (85332-2-RR, Clone: 242218E4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85332-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85332-2-RR

    Affinity and Kinetic Characterization of 85332-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85332-2-RR against Human UBE4B were performed. The affinity constant is 0.895 nM.

UBE4B Recombinant antibody, PBS Only

UBE4B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85332-2-PBS

产品说明书

CloneNo. 242218E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

EC:2.3.2.27, HDNB1, KIAA0684, UBOX3, UFD2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy UBE4B Ready-To-Use IHC Kit. UBE4B primary antibody included.

产品信息

85332-2-PBS targets UBE4B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 UBE4B fusion protein Ag12836 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitination factor E4B (UFD2 homolog, yeast)
别名 EC:2.3.2.27, HDNB1, KIAA0684, UBOX3, UFD2
计算分子量 1173 aa, 133 kDa
观测分子量140-146 kDa
GenBank蛋白编号BC093696
基因名称 UBE4B
Gene ID (NCBI) 10277
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95155
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.