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验证数据展示

  • WB analysis of human testis using 16928-1-AP

    WB analysis of human testis using 16928-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16928-1-AP (UBE2Z antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 16928-1-AP

    IHC staining of human placenta using 16928-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16928-1-AP (UBE2Z antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 16928-1-AP

    IHC staining of human placenta using 16928-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16928-1-AP (UBE2Z antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBE2Z Polyclonal antibody

UBE2Z Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16928-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

E2 ubiquitin-conjugating enzyme Z, EC:2.3.2.23, HOYS7, Uba6-specific E2 conjugating enzyme 1, Ubiquitin carrier protein Z

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse pancreas tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16928-1-AP targets UBE2Z in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10347

Product name: Recombinant human UBE2Z protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-241 aa of BC015169

Sequence: EPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRFNPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKCPCPEPLRGVMEKSFLEYYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2Z
别名 E2 ubiquitin-conjugating enzyme Z, EC:2.3.2.23, HOYS7, Uba6-specific E2 conjugating enzyme 1, Ubiquitin carrier protein Z
计算分子量 28 kDa, 38 kDa
观测分子量 45 kDa
GenBank蛋白编号BC015169
基因名称 UBE2Z
Gene ID (NCBI) 65264
RRIDAB_2211782
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H832
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for UBE2Z antibody 16928-1-APDownload protocol
WB protocol for UBE2Z antibody 16928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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