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验证数据展示

  • WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

    WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81854-1-RR (UBE2S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

    IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

    IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using UBE2S antibody (81854-1-RR, Clone: 6D18 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81854-1-RR

    Affinity and Kinetic Characterization of 81854-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81854-1-RR against Human UBE2S were performed. The affinity constant is below 1 pM.

UBE2S Recombinant monoclonal antibody, PBS Only

UBE2S Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81854-1-PBS

产品说明书

CloneNo. 6D18

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

6D18, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

81854-1-PBS targets UBE2S in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5264

Product name: Recombinant human UBE2S protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-222 aa of BC066948

Sequence: MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHLNVGANGEICVNVLKRDWTAELGIRHVLLTIRCLLIHPNPESALNEEAGRLLLENSEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTAASSTDPGAPGGLGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2S
别名 6D18, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23
计算分子量 222 aa, 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC066948
基因名称 UBE2S
Gene ID (NCBI) 27338
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16763
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

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