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验证数据展示

  • WB analysis of HEK-293 using 66897-1-Ig

    WB analysis of HEK-293 using 66897-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

UBE2S Monoclonal antibody

UBE2S Monoclonal Antibody for WB, ELISA
Cat No. 66897-1-Ig

产品说明书

CloneNo. 8C9E6

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, ELISA

别名

8C9E6, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66897-1-Ig targets UBE2S in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5378

Product name: Recombinant human UBE2S protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-222 aa of BC066948

Sequence: MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHLNVGANGEICVNVLKRDWTAELGIRHVLLTIRCLLIHPNPESALNEEAGRLLLENSEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTAASSTDPGAPGGLGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2S
别名 8C9E6, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23
计算分子量 222 aa, 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC066948
基因名称 UBE2S
Gene ID (NCBI) 27338
RRIDAB_2882226
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ16763
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

实验方案

Product Specific Protocols
WB protocol for UBE2S antibody 66897-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols