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验证数据展示

  • WB analysis of HL-60 using 18219-1-AP

    WB analysis of HL-60 using 18219-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 18219-1-AP

    IHC staining of human skin cancer using 18219-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18219-1-AP (UBE2S antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 18219-1-AP

    IHC staining of human ovary cancer using 18219-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18219-1-AP (UBE2S antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBE2S Polyclonal antibody

UBE2S Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18219-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, COLO 320 cells, MCF-7 cells, MDA-MB-453s cells, mouse ovary tissue
Positive IHC detected inhuman skin cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18219-1-AP targets UBE2S in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 UBE2S fusion protein Ag12978 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2S
别名 OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF
计算分子量 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC004236
基因名称 UBE2S
Gene ID (NCBI) 27338
RRIDAB_10598771
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16763
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

实验方案

Product Specific Protocols
WB protocol for UBE2S antibody 18219-1-APDownload protocol
IHC protocol for UBE2S antibody 18219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols