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验证数据展示

  • WB analysis of Jurkat using 14077-1-AP

    WB analysis of Jurkat using 14077-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14077-1-AP (UBE2R2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 14077-1-AP

    IHC staining of human spleen using 14077-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14077-1-AP (UBE2R2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human spleen using 14077-1-AP

    IHC staining of human spleen using 14077-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14077-1-AP (UBE2R2 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 14077-1-AP

    IF Staining of HeLa using 14077-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14077-1-AP (UBE2R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

UBE2R2 Polyclonal antibody

UBE2R2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14077-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Ubiquitin protein ligase R2, Ubiquitin carrier protein R2, UBC3B, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme R2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse spleen tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

14077-1-AP targets UBE2R2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 UBE2R2 fusion protein Ag5202 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2R 2
别名 Ubiquitin protein ligase R2, Ubiquitin carrier protein R2, UBC3B, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme R2
计算分子量 27 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC047584
基因名称 UBE2R2
Gene ID (NCBI) 54926
RRIDAB_2878010
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ712K3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2R2 antibody 14077-1-APDownload protocol
IHC protocol for UBE2R2 antibody 14077-1-APDownload protocol
IF protocol for UBE2R2 antibody 14077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Shared and distinct mechanisms of UBA1 inactivation across different diseases

Authors - Jason C Collins
View Article
mouseWB

JCI Insight

PMID: 31292296

Authors - Isabelle K Vila
View Article
humanWB

bioRxiv

Shared and Distinct Mechanisms of UBA1 Inactivation Across Different Diseases

Authors - Jason C Collins
View Article
humanWB

Exp Gerontol

p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6

Authors - Ying Cheng
View Article