验证数据展示
经过测试的应用
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16072-1-AP targets UBE2Q1 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9028 Product name: Recombinant human UBE2Q1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 258-422 aa of BC009286 Sequence: KELRDIYRSQSFKGGNYAVELVNDSLYDWNVKLLKVDQDSALHNDLQILKEKEGADFILLNFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGWSSAYSIESVIMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTPPKEDG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-conjugating enzyme E2Q family member 1 |
| 别名 | E2 ubiquitin-conjugating enzyme Q1, EC:2.3.2.23, GTAP, NICE 5, NICE5 |
| 计算分子量 | 422 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC009286 |
| 基因名称 | UBE2Q1 |
| Gene ID (NCBI) | 55585 |
| RRID | AB_3741876 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z7E8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBE2Q1 is a member of the ubiquitin-conjugating enzyme E2 family that plays key regulatory roles in various physiological and pathological processes, including cell proliferation, tumorigenesis, and reproductive development, by catalyzing the ubiquitination of substrates.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for UBE2Q1 antibody 16072-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
