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验证数据展示

  • WB analysis of HeLa using 15812-1-AP (same clone as 15812-1-PBS)

    WB analysis of HeLa using 15812-1-AP (same clone as 15812-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15812-1-AP (UBE2O antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15812-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 15812-1-AP (same clone as 15812-1-PBS)

    IHC staining of human heart using 15812-1-AP (same clone as 15812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15812-1-AP (UBE2O Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15812-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 15812-1-AP (same clone as 15812-1-PBS)

    IHC staining of human heart using 15812-1-AP (same clone as 15812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15812-1-AP (UBE2O Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15812-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UBE2O Polyclonal antibody, PBS Only

UBE2O Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15812-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15812-1-PBS targets UBE2O in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8580

Product name: Recombinant human UBE2O protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-343 aa of BC007822

Sequence: SSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2O
别名
计算分子量 1292 aa, 141 kDa
观测分子量 150 kDa, 200-230 kDa
GenBank蛋白编号BC007822
基因名称 UBE2O
Gene ID (NCBI) 63893
RRIDAB_2211192
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0C9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2O, also named as KIAA1734, belongs to the ubiquitin-conjugating enzyme family. It catalyzes the covalent attachment of ubiquitin to other proteins. UBE2O has a calculated molecular mass of 141 kDa, and many bands can be detected as 140-170 kDa and 200-230 kDa (PMID: 28774900, 23455153, 28774922, 11311559).

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