验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse testis tissue, human brain tissue, rat brain tissue, rat testis tissue |
| Positive IHC detected in | human gliomas tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
11834-3-AP targets UBE2K in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag2415 Product name: Recombinant human UBE2K protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC022804 Sequence: MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) |
| 别名 | E2-25K, HIP 2, HIP2, HYPG, LIG, UBE2K, Ubiquitin carrier protein, Ubiquitin protein ligase |
| 计算分子量 | 200 aa, 22 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC022804 |
| 基因名称 | UBE2K |
| Gene ID (NCBI) | 3093 |
| RRID | AB_2241168 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61086 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBE2K antibody 11834-3-AP | Download protocol |
| WB protocol for UBE2K antibody 11834-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Oncol UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma | ||
Biochem Biophys Res Commun Ixazomib inhibits myeloma cell proliferation by targeting UBE2K. | ||
Biomed Environ Sci Ezrin Promotes the Proliferation, Migration, and Invasion of Ovarian Cancer Cells. | ||
Neuropathology Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. |
