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验证数据展示

  • WB analysis using 10722-1-AP

    WB analysis using 10722-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 10722-1-AP

    IHC staining of mouse testis using 10722-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10722-1-AP (UBE2G2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBE2G2 Polyclonal antibody

UBE2G2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10722-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

E2 ubiquitin-conjugating enzyme G2, EC:2.3.2.23, UBC 7, UBC7, UBE2G 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10722-1-AP targets UBE2G2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1082

Product name: Recombinant human UBE2G2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-165 aa of BC008351

Sequence: MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)
别名 E2 ubiquitin-conjugating enzyme G2, EC:2.3.2.23, UBC 7, UBC7, UBE2G 2
计算分子量 19 kDa
观测分子量 16-19 kDa
GenBank蛋白编号BC008351
基因名称 UBE2G2
Gene ID (NCBI) 7327
RRIDAB_2256726
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60604
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2G2 antibody 10722-1-APDownload protocol
IHC protocol for UBE2G2 antibody 10722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols