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验证数据展示

  • WB analysis using 17056-1-AP (same clone as 17056-1-PBS)

    WB analysis using 17056-1-AP (same clone as 17056-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

    IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

    IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 17056-1-AP (same clone as 17056-1-PBS)

    IF Staining of NIH/3T3 using 17056-1-AP (same clone as 17056-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 17056-1-AP (UBE2F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

UBE2F Polyclonal antibody, PBS Only

UBE2F Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17056-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17056-1-PBS targets UBE2F in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10764

Product name: Recombinant human UBE2F protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-185 aa of BC010549

Sequence: MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHLRDKEDFRNKVDDYIKRYAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2F (putative)
别名
计算分子量 185 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC010549
基因名称 UBE2F
Gene ID (NCBI) 140739
RRIDAB_2210295
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969M7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2F(Ubiquitin-conjugating enzyme E2 F) is also named as NCE2(NEDD8-conjugating enzyme 2) and belongs to the ubiquitin-conjugating enzyme family.The canonical uBE2M catalyses the modification of most cullins, whereas uBE2F is specifically responsible for cuL5 neddylation. uBE2F is regulated differently than uBE2M, supporting the idea that neddylation is used to fine-tune ubiquitin-chain formation by different cullin-dependent E3s(PMID:19465892).

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