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- KD/KO Validated
UBE2E1 Polyclonal antibody
UBE2E1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55457-1-AP targets UBE2E1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast) |
Synonyms | UBCH6, UBE2E1, Ubiquitin carrier protein E1, Ubiquitin protein ligase E1 |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 21-27 kDa |
GenBank Accession Number | NM_003341 |
Gene Symbol | UBE2E1 |
Gene ID (NCBI) | 7324 |
RRID | AB_2918357 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P51965 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for UBE2E1 antibody 55457-1-AP | Download protocol |
IHC protocol for UBE2E1 antibody 55457-1-AP | Download protocol |
IF protocol for UBE2E1 antibody 55457-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |