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UBC9 Monoclonal antibody

UBC9 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (1)

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4A10B11

Cat No : 60201-1-Ig

Print datasheet

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

验证数据展示

  • WB analysis using 60201-1-Ig

    WB analysis using 60201-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60201-1-Ig (UBC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 60201-1-Ig

    IHC staining of human lymphoma using 60201-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2I/UBC9 antibody included
  • IHC staining of human lymphoma using 60201-1-Ig

    IHC staining of human lymphoma using 60201-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2I/UBC9 antibody included
  • FC experiment of Jurkat using 60201-1-Ig

    FC experiment of Jurkat using 60201-1-Ig

    1X10^6 Jurkat cells were stained with 0.20ug UBC9 antibody (60201-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

经过测试的应用

Positive WB detected inLNCaP cells, rat kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy UBE2I/UBC9 Ready-To-Use IHC Kit. UBE2I/UBC9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:600
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 60201-1-Ig is UBC9 Fusion Protein expressed in E. coli.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, monkey
Immunogen UBC9 fusion protein Ag7885 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Synonyms C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC004437
Gene Symbol UBE2I
Gene ID (NCBI) 7329
RRIDAB_10918977
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

实验方案

Product Specific Protocols
WB protocol for UBC9 antibody 60201-1-IgDownload protocol
IHC protocol for UBC9 antibody 60201-1-IgDownload protocol
FC protocol for UBC9 antibody 60201-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,monkeyWB,IF

Viruses

The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation

Authors - Congcong Huang
View Article