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UBC9 Polyclonal antibody

UBC9 Polyclonal Antibody for WB, IP, ELISA
Cat No. 51018-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IP, ELISA

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse thymus tissue
Positive IP detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy UBE2I/UBC9 Ready-To-Use IHC Kit. UBE2I/UBC9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51018-2-AP targets UBC9 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
别名 C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC051289
基因名称 UBC9
Gene ID (NCBI) 7329
RRIDAB_2210591
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63279
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

实验方案

Product Specific Protocols
WB protocol for UBC9 antibody 51018-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin

Authors - Tao Zhang
human

Autophagy

PDPK1 regulates autophagosome biogenesis by binding to PIK3C3.

Authors - Boli Hu
mouseWB

PLoS Genet

IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.

Authors - Bo Chen
pigWB

Front Immunol

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Authors - Xu Ye
humanWB

J Biol Chem

Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.

Authors - Tao Wang
  • KD Validated
humanWB

MBio

Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications.

Authors - Sook-Young Sohn
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