• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67482-1-Ig (same clone as 67482-1-PBS)

    WB analysis using 67482-1-Ig (same clone as 67482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67482-1-Ig (UBC12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67482-1-Ig (same clone as 67482-1-PBS)

    WB analysis of HEK-293 using 67482-1-Ig (same clone as 67482-1-PBS)

    WB result of UBC12 antibody (67482-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67482-1-Ig (same clone as 67482-1-PBS)

    IF Staining of HeLa using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC12 antibody (67482-1-Ig, Clone: 1H8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

UBC12 Monoclonal antibody, PBS Only

UBC12 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67482-1-PBS

产品说明书

CloneNo. 1H8D2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

UBE2M, EC:2.3.2.34, hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

67482-1-PBS targets UBC12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 UBC12 fusion protein Ag5984 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
别名 UBE2M, EC:2.3.2.34, hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC058924
基因名称 UBC12
Gene ID (NCBI) 9040
RRIDAB_2882709
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61081
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C