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验证数据展示

  • WB analysis using 14520-1-AP

    WB analysis using 14520-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 14520-1-AP

    WB analysis of HEK-293 using 14520-1-AP

    WB result of UBC12 antibody (14520-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells.

  • IHC staining of mouse ovary using 14520-1-AP

    IHC staining of mouse ovary using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IHC staining of mouse ovary using 14520-1-AP

    IHC staining of mouse ovary using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IP experiment of L02 using 14520-1-AP

    IP experiment of L02 using 14520-1-AP

    IP result of anti-UBC12 (IP:14520-1-AP, 4ug; Detection:14520-1-AP 1:600) with L02 cells lysate 1800ug.

  • IF Staining of L02 using 14520-1-AP

    IF Staining of L02 using 14520-1-AP

    Immunofluorescent analysis of L02 cells using 14520-1-AP (UBC12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 14520-1-AP

    IHC staining of human colon cancer using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IHC staining of human placenta using 14520-1-AP

    IHC staining of human placenta using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IHC staining of rat brain using 14520-1-AP

    IHC staining of rat brain using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IHC staining of rat colon using 14520-1-AP

    IHC staining of rat colon using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IHC staining of rat lung using 14520-1-AP

    IHC staining of rat lung using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IHC staining of human placenta using 14520-1-AP

    IHC staining of human placenta using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IHC staining of rat brain using 14520-1-AP

    IHC staining of rat brain using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • IHC staining of rat brain using 14520-1-AP

    IHC staining of rat brain using 14520-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBE2M/UBC12 antibody included
  • Featured Product
  • KD/KO Validated

UBC12 Polyclonal antibody

UBC12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14520-1-AP

产品说明书

引用文献(21)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

UBE2M, EC:2.3.2.34, hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, L02 cells, SMMC-7721 cells, HEK-293 cells, Ramos cells
Positive IP detected inL02 cells
Positive IHC detected inmouse ovary tissue, human placenta tissue, rat lung tissue, rat colon tissue, human colon cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells
Planning an IHC experiment? We recommend our IHCeasy® UBE2M/UBC12 Ready-To-Use IHC Kit. UBE2M/UBC12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14520-1-AP targets UBC12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, monkey
免疫原

CatNo: Ag5984

Product name: Recombinant human UBC12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-183 aa of BC058924

Sequence: MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
别名 UBE2M, EC:2.3.2.34, hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC058924
基因名称 UBC12
Gene ID (NCBI) 9040
RRIDAB_2878063
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61081
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for UBC12 antibody 14520-1-APDownload protocol
IHC protocol for UBC12 antibody 14520-1-APDownload protocol
IP protocol for UBC12 antibody 14520-1-APDownload protocol
WB protocol for UBC12 antibody 14520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.

Authors - Haibin Zhou
View Article
humanIHC

J Exp Clin Cancer Res

Neddylation activated TRIM25 desensitizes triple-negative breast cancer to paclitaxel via TFEB-mediated autophagy

Authors - Bowen Zheng
View Article
mouse,humanWB,IP

Adv Sci (Weinh)

Neddylation Targets and Stabilizes NLRP3 to Augment Inflammasome-Mediated Colitis and Mood Disorder.

Authors - Wenbin Gai
View Article
humanWB

Cancer Biol Med

CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.

Authors - Ting Li
View Article
humanWB,IP,IHC,IF

Cell Death Dis

PRMT1-mediated methylation of UBE2m promoting calcium oxalate crystal-induced kidney injury by inhibiting fatty acid metabolism.

Authors - Tianhui Yuan
View Article
humanIF

Pharmacol Res

Orlistat targets NEDD8 conjugating enzyme UBC12 for cancer therapy.

Authors - Mengzhen Shen
View Article
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