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验证数据展示

  • WB analysis using 15823-1-AP (same clone as 15823-1-PBS)

    WB analysis using 15823-1-AP (same clone as 15823-1-PBS)

    mouse bone marrow tissue were subjected to SDS PAGE followed by western blot with 15823-1-AP (UBASH3A-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse spleen using 15823-1-AP (same clone as 15823-1-PBS)

    IP experiment of mouse spleen using 15823-1-AP (same clone as 15823-1-PBS)

    IP result of anti-UBASH3A-Specific (IP:15823-1-AP, 4ug; Detection:15823-1-AP 1:500) with mouse spleen tissue lysate 1920 ug. This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 15823-1-AP (same clone as 15823-1-PBS)

    IF Staining of THP-1 using 15823-1-AP (same clone as 15823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using UBASH3A-Specific antibody (15823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

UBASH3A-Specific Polyclonal antibody, PBS Only

UBASH3A-Specific Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 15823-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15823-1-PBS targets UBASH3A-Specific in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin associated and SH3 domain containing, A
别名
计算分子量 74 kDa
观测分子量 70-74 kDa
GenBank蛋白编号NM_018961
基因名称 UBASH3A
Gene ID (NCBI) 53347
RRIDAB_2272584
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57075
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBASH3A gene encodes ubiquitin-associated and SH3 domain-containing protein A (UBASH3A) also known as T-cell ubiquitin ligand 1 (TULA1) which is one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. UBASH3A gene expression is limited to only a few tissues, with its highest expression in spleen, peripheral blood leukocytes, and bone marrow. UBASH3A protein is associated with c-Cbl and ubiquitylated proteins and has been implicated in the regulation of signaling mediated by protein-tyrosine kinases oth family members can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells via its interaction with apoptosis-inducing factor (AIF). This antibody is UBASH3A-specific.

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