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验证数据展示

  • WB analysis using 29628-1-AP

    WB analysis using 29628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29628-1-AP (UBAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 29628-1-AP

    IF Staining of U2OS using 29628-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using UBAP2 antibody (29628-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

UBAP2 Polyclonal antibody

UBAP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29628-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

bA176F3.5, KIAA1491, UBAP 2, ubiquitin associated protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29628-1-AP targets UBAP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30572

Product name: Recombinant human UBAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 395-515 aa of NM_018449

Sequence: QQNSTSHPTTTTSWDLKPPTSQSSVLSHLDFKSQPEPSPVLSQLSQRQQHQSQAVTVPPPGLESFPSQAKLRESTPGDSPSTVNKLLQLPSTTIENISVSVHQPQPKHIKLAKRRIPPASK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin associated protein 2
别名 bA176F3.5, KIAA1491, UBAP 2, ubiquitin associated protein 2
计算分子量 117 kDa
观测分子量117 kDa
GenBank蛋白编号NM_018449
基因名称 UBAP2
Gene ID (NCBI) 55833
RRIDAB_3086147
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T6F2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for UBAP2 antibody 29628-1-APDownload protocol
WB protocol for UBAP2 antibody 29628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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