- Featured Product
- KD/KO Validated
UBAP1 Polyclonal antibody
UBAP1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human heart tissue, A431 cells, human lung tissue, human spleen tissue, human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, mouse thymus tissue, Y79 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human lung cancer tissue, human heart tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12385-1-AP targets UBAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | UBAP1 fusion protein Ag3055 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin associated protein 1 |
Synonyms | NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1 |
Calculated Molecular Weight | 502 aa, 55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC020950 |
Gene Symbol | UBAP1 |
Gene ID (NCBI) | 51271 |
RRID | AB_2211886 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NZ09 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for UBAP1 antibody 12385-1-AP | Download protocol |
IHC protocol for UBAP1 antibody 12385-1-AP | Download protocol |
IF protocol for UBAP1 antibody 12385-1-AP | Download protocol |
IP protocol for UBAP1 antibody 12385-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EMBO J A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors. | ||
Curr Biol UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. | ||
Cell Rep The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery. | ||
Cell Rep Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration. | ||
PLoS Pathog A cytoplasmic tail determinant in HIV-1 Vpu mediates targeting of tetherin for endosomal degradation and counteracts interferon-induced restriction. |