验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HEK-293 cells, mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:3000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25122-1-AP targets UBAC2 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | UBAC2 fusion protein Ag19142 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UBA domain containing 2 |
| 别名 | Ubiquitin-associated domain-containing protein 2, UBAC 2, UBA domain-containing protein 2, PSEC0110, Phosphoglycerate dehydrogenase-like protein 1 |
| 计算分子量 | 344 aa, 39 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC121139 |
| 基因名称 | UBAC2 |
| Gene ID (NCBI) | 337867 |
| RRID | AB_2879910 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NBM4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBAC2 antibody 25122-1-AP | Download protocol |
| IHC protocol for UBAC2 antibody 25122-1-AP | Download protocol |
| IF protocol for UBAC2 antibody 25122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells. | ||
Cell Death Dis UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis.
| ||
Mol Cell TMUB1 is an endoplasmic reticulum-resident escortase that promotes the p97-mediated extraction of membrane proteins for degradation | ||
