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验证数据展示

  • WB analysis of MDA-MB-453s using 25262-1-AP (same clone as 25262-1-PBS)

    WB analysis of MDA-MB-453s using 25262-1-AP (same clone as 25262-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 25262-1-AP (same clone as 25262-1-PBS)

    IHC staining of human ovary cancer using 25262-1-AP (same clone as 25262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 25262-1-AP (same clone as 25262-1-PBS)

    IF Staining of HepG2 using 25262-1-AP (same clone as 25262-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 25262-1-AP (UAP1L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 25262-1-AP (same clone as 25262-1-PBS)

    IHC staining of human ovary cancer using 25262-1-AP (same clone as 25262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

UAP1L1 Polyclonal antibody, PBS Only

UAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25262-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:2.7.7.-, UDP-N-acetylhexosamine pyrophosphorylase-like protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25262-1-PBS targets UAP1L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-N-acteylglucosamine pyrophosphorylase 1-like 1
别名 EC:2.7.7.-, UDP-N-acetylhexosamine pyrophosphorylase-like protein 1
计算分子量 57 kDa
观测分子量 57-59 kDa
GenBank蛋白编号NM_207309
基因名称 UAP1L1
Gene ID (NCBI) 91373
RRIDAB_2879993
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KQV9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UDP-N-acetylglucosamine pyrophosphorylase-1-like-1 (UAP1L1) is a protein that shares ~59% sequence identity with UDP-N-acetylglucosamine pryophosphorylase-1 (UAP1), which is directly involved in the synthesis of the sugar donor (UDP-GlcNac) for N-acetylglucosamine modification (O-GlcNAclation) of proteins in the cytoplasm, nucleus, and mitochondria, as well as for O- and N-linked protein glycosylation in the endoplasmic reticulum and the Golgi apparatus (PMID: 30097606, 35168617). Human UAP1L1 is also significantly upregulated in a distinct subset of HCC tissues and patients with upregulated expression of UAP1L1 appeared to have poor prognosis (PMID: 33987240).

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